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cd4+ t cell isolation kit, mouse  (Miltenyi Biotec)


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    Miltenyi Biotec cd4+ t cell isolation kit, mouse
    Cd4+ T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+t+cell+isolation/CD4%2B+T+Cell+Isolation+Kit%2C+mouse/custom%40130-104-454%4042810527
    Average 97 stars, based on 504 article reviews
    cd4+ t cell isolation kit, mouse - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Enhanced Therapeutic Effect of IL-10-ADSCs on Rabbit Autoimmune Dacryoadenitis By Suppressing T Follicular Helper Cell Responses Via miR-142-5p/RC3H1 Axis.
    Article Snippet: .. CD4+T Cell Isolation and Tfh Cell Differentiation Naïve CD4+T cells were purified from PBMC samples obtained from healthy volunteers using magnetic bead negative selection with the Naïve CD4+T cell Isolation Kit (Miltenyi Biotec, Germany), according to the manufacturer’s instructions. .. Fibroblasts (HDF-α cell line, Stem Cell Bank, Chinese Academy of Sciences), IL-10-ADSCs or ADSCs were co-cultured with the naïve CD4+T cells at a ratio of 1:5 for a period of 5 days, under Tfh cell-polarizing conditions: Human CD3/CD28 T Cell Activator (25 μL/mL; Stemcell Technologies, Vancouver, BC, Canada), recombinant humanmouse-rat activin A (100 ng/mL; R&D Systems, Minneapolis, MN, USA), and recombinant human IL-12 (5 ng/mL; R&D Systems, Minneapolis, MN, USA).

    Article Title: Aged mice-derived bronchial epithelial cells regulate Th17 cell differentiation in asthma via the MBD2-sICOSL axis.
    Article Snippet: Th17 cells are involved in the pathogenesis of elderly asthma.. Bronchial epithelial cells (BECs) can act as antigenpresenting cells, and our previous studies have shown that methyl-CPG binding domain protein 2 (MBD2) in BECs can promote Th17 cell differentiation in asthma.. However, the effect of BECs from different age groups (young and old) on Th17 cells remains unclear.

    Article Title: Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites.
    Article Snippet: Electrophoretic mobility shift assay TnG repeats DNA (0.2 mM) was mixed with the indicated amount of FoxP3DN and mutations in the EMSA buffer A (20mM HEPES pH 7.5, 150 mM NaCl, 1.5 mMMgCl2 and 2 mM DTT), incubated for 30 min at 4 C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 C. After staining with Sybr Gold stain (Life Technologies), Sybr Gold fluorescence was recorded using ChemiDoc Imaging Systems (Bio-Rad) and analyzed with ChemiDoc Analysis Software. .. CD4+ T cell isolation and retroviral transduction Naı̈ve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction. .. The purity was estimated to be >90% as measured by PE anti-CD4 (Biolegend) staining and FACS analysis.

    Article Title: Functional Dichotomy of Developmental Foxp3 + Treg Cell Subsets in the Visceral Adipose Tissue of Lean and Obese Mice
    Article Snippet: Viable cell numbers were determined using propidium iodide and a MACSQuant (Miltenyi Biotec). .. Prior to CD4 + T cell isolation from VAT via FACS, samples were depleted of lineage marker-positive cells (CD8a, CD11b, CD11c, CD14, and CD19) using biotin-conjugated mAbs, along with streptavidin-conjugated microbeads and the autoMACS Pro magnetic separation system (Miltenyi Biotec). .. Samples were stained with DRAQ7 (BioStatus) for dead cell exclusion, filtered through 40 μm cell strainers, and analyzed on an LSR Fortessa or sorted with a FACS Aria II or III (all BD).

    Article Title: DKK3 Initially Preserves Acinar Integrity Through MEK-Fos Signaling, but Later Switches to an Oncogenic Role in Pancreatic Cancer.
    Article Snippet: .. [48] and subsequent CD4+ T cell isolation was performed using CD4+ T Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s protocol. .. Prior to the start of co-culture, CD4+ T cells were activated for 24 h using Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher Scientific).


    Article Title: Multi-omics uncovers transcriptional programs of gut-resident memory CD4 + T cells in Crohn’s disease
    Article Snippet: .. Isolated PBMCs were then used for CD4 + T cell isolation with CD4 + T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol. ..

    Article Title: Aged regulatory T cells fail to control autoimmune lacrimal gland pathogenic CD4 + T cells.
    Article Snippet: CD25KO mice are a model of Sjögren disease.. CD25KO mice have severe inflammation and infiltrating lymphocytes to the lacrimal glands (LG).. Whether the pathogenicity of CD25KO CD4+ T cells can be controlled in vivo by Tregs is unknown.

    Cell Differentiation:

    Article Title: Enhanced Therapeutic Effect of IL-10-ADSCs on Rabbit Autoimmune Dacryoadenitis By Suppressing T Follicular Helper Cell Responses Via miR-142-5p/RC3H1 Axis.
    Article Snippet: .. CD4+T Cell Isolation and Tfh Cell Differentiation Naïve CD4+T cells were purified from PBMC samples obtained from healthy volunteers using magnetic bead negative selection with the Naïve CD4+T cell Isolation Kit (Miltenyi Biotec, Germany), according to the manufacturer’s instructions. .. Fibroblasts (HDF-α cell line, Stem Cell Bank, Chinese Academy of Sciences), IL-10-ADSCs or ADSCs were co-cultured with the naïve CD4+T cells at a ratio of 1:5 for a period of 5 days, under Tfh cell-polarizing conditions: Human CD3/CD28 T Cell Activator (25 μL/mL; Stemcell Technologies, Vancouver, BC, Canada), recombinant humanmouse-rat activin A (100 ng/mL; R&D Systems, Minneapolis, MN, USA), and recombinant human IL-12 (5 ng/mL; R&D Systems, Minneapolis, MN, USA).

    Purification:

    Article Title: Enhanced Therapeutic Effect of IL-10-ADSCs on Rabbit Autoimmune Dacryoadenitis By Suppressing T Follicular Helper Cell Responses Via miR-142-5p/RC3H1 Axis.
    Article Snippet: .. CD4+T Cell Isolation and Tfh Cell Differentiation Naïve CD4+T cells were purified from PBMC samples obtained from healthy volunteers using magnetic bead negative selection with the Naïve CD4+T cell Isolation Kit (Miltenyi Biotec, Germany), according to the manufacturer’s instructions. .. Fibroblasts (HDF-α cell line, Stem Cell Bank, Chinese Academy of Sciences), IL-10-ADSCs or ADSCs were co-cultured with the naïve CD4+T cells at a ratio of 1:5 for a period of 5 days, under Tfh cell-polarizing conditions: Human CD3/CD28 T Cell Activator (25 μL/mL; Stemcell Technologies, Vancouver, BC, Canada), recombinant humanmouse-rat activin A (100 ng/mL; R&D Systems, Minneapolis, MN, USA), and recombinant human IL-12 (5 ng/mL; R&D Systems, Minneapolis, MN, USA).

    Selection:

    Article Title: Enhanced Therapeutic Effect of IL-10-ADSCs on Rabbit Autoimmune Dacryoadenitis By Suppressing T Follicular Helper Cell Responses Via miR-142-5p/RC3H1 Axis.
    Article Snippet: .. CD4+T Cell Isolation and Tfh Cell Differentiation Naïve CD4+T cells were purified from PBMC samples obtained from healthy volunteers using magnetic bead negative selection with the Naïve CD4+T cell Isolation Kit (Miltenyi Biotec, Germany), according to the manufacturer’s instructions. .. Fibroblasts (HDF-α cell line, Stem Cell Bank, Chinese Academy of Sciences), IL-10-ADSCs or ADSCs were co-cultured with the naïve CD4+T cells at a ratio of 1:5 for a period of 5 days, under Tfh cell-polarizing conditions: Human CD3/CD28 T Cell Activator (25 μL/mL; Stemcell Technologies, Vancouver, BC, Canada), recombinant humanmouse-rat activin A (100 ng/mL; R&D Systems, Minneapolis, MN, USA), and recombinant human IL-12 (5 ng/mL; R&D Systems, Minneapolis, MN, USA).

    Article Title: Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites.
    Article Snippet: Electrophoretic mobility shift assay TnG repeats DNA (0.2 mM) was mixed with the indicated amount of FoxP3DN and mutations in the EMSA buffer A (20mM HEPES pH 7.5, 150 mM NaCl, 1.5 mMMgCl2 and 2 mM DTT), incubated for 30 min at 4 C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 C. After staining with Sybr Gold stain (Life Technologies), Sybr Gold fluorescence was recorded using ChemiDoc Imaging Systems (Bio-Rad) and analyzed with ChemiDoc Analysis Software. .. CD4+ T cell isolation and retroviral transduction Naı̈ve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction. .. The purity was estimated to be >90% as measured by PE anti-CD4 (Biolegend) staining and FACS analysis.

    Article Title: Aged regulatory T cells fail to control autoimmune lacrimal gland pathogenic CD4 + T cells.
    Article Snippet: CD25KO mice are a model of Sjögren disease.. CD25KO mice have severe inflammation and infiltrating lymphocytes to the lacrimal glands (LG).. Whether the pathogenicity of CD25KO CD4+ T cells can be controlled in vivo by Tregs is unknown.

    Isolation:

    Article Title: Aged mice-derived bronchial epithelial cells regulate Th17 cell differentiation in asthma via the MBD2-sICOSL axis.
    Article Snippet: Th17 cells are involved in the pathogenesis of elderly asthma.. Bronchial epithelial cells (BECs) can act as antigenpresenting cells, and our previous studies have shown that methyl-CPG binding domain protein 2 (MBD2) in BECs can promote Th17 cell differentiation in asthma.. However, the effect of BECs from different age groups (young and old) on Th17 cells remains unclear.

    Article Title: Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites.
    Article Snippet: Electrophoretic mobility shift assay TnG repeats DNA (0.2 mM) was mixed with the indicated amount of FoxP3DN and mutations in the EMSA buffer A (20mM HEPES pH 7.5, 150 mM NaCl, 1.5 mMMgCl2 and 2 mM DTT), incubated for 30 min at 4 C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 C. After staining with Sybr Gold stain (Life Technologies), Sybr Gold fluorescence was recorded using ChemiDoc Imaging Systems (Bio-Rad) and analyzed with ChemiDoc Analysis Software. .. CD4+ T cell isolation and retroviral transduction Naı̈ve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction. .. The purity was estimated to be >90% as measured by PE anti-CD4 (Biolegend) staining and FACS analysis.

    Article Title: Multi-omics uncovers transcriptional programs of gut-resident memory CD4 + T cells in Crohn’s disease
    Article Snippet: .. Isolated PBMCs were then used for CD4 + T cell isolation with CD4 + T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol. ..

    Article Title: Aged regulatory T cells fail to control autoimmune lacrimal gland pathogenic CD4 + T cells.
    Article Snippet: CD25KO mice are a model of Sjögren disease.. CD25KO mice have severe inflammation and infiltrating lymphocytes to the lacrimal glands (LG).. Whether the pathogenicity of CD25KO CD4+ T cells can be controlled in vivo by Tregs is unknown.

    Retroviral:

    Article Title: Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites.
    Article Snippet: Electrophoretic mobility shift assay TnG repeats DNA (0.2 mM) was mixed with the indicated amount of FoxP3DN and mutations in the EMSA buffer A (20mM HEPES pH 7.5, 150 mM NaCl, 1.5 mMMgCl2 and 2 mM DTT), incubated for 30 min at 4 C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 C. After staining with Sybr Gold stain (Life Technologies), Sybr Gold fluorescence was recorded using ChemiDoc Imaging Systems (Bio-Rad) and analyzed with ChemiDoc Analysis Software. .. CD4+ T cell isolation and retroviral transduction Naı̈ve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction. .. The purity was estimated to be >90% as measured by PE anti-CD4 (Biolegend) staining and FACS analysis.

    Transduction:

    Article Title: Ultrastable and versatile multimeric ensembles of FoxP3 on microsatellites.
    Article Snippet: Electrophoretic mobility shift assay TnG repeats DNA (0.2 mM) was mixed with the indicated amount of FoxP3DN and mutations in the EMSA buffer A (20mM HEPES pH 7.5, 150 mM NaCl, 1.5 mMMgCl2 and 2 mM DTT), incubated for 30 min at 4 C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 C. After staining with Sybr Gold stain (Life Technologies), Sybr Gold fluorescence was recorded using ChemiDoc Imaging Systems (Bio-Rad) and analyzed with ChemiDoc Analysis Software. .. CD4+ T cell isolation and retroviral transduction Naı̈ve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction. .. The purity was estimated to be >90% as measured by PE anti-CD4 (Biolegend) staining and FACS analysis.

    FACS:

    Article Title: Functional Dichotomy of Developmental Foxp3 + Treg Cell Subsets in the Visceral Adipose Tissue of Lean and Obese Mice
    Article Snippet: Viable cell numbers were determined using propidium iodide and a MACSQuant (Miltenyi Biotec). .. Prior to CD4 + T cell isolation from VAT via FACS, samples were depleted of lineage marker-positive cells (CD8a, CD11b, CD11c, CD14, and CD19) using biotin-conjugated mAbs, along with streptavidin-conjugated microbeads and the autoMACS Pro magnetic separation system (Miltenyi Biotec). .. Samples were stained with DRAQ7 (BioStatus) for dead cell exclusion, filtered through 40 μm cell strainers, and analyzed on an LSR Fortessa or sorted with a FACS Aria II or III (all BD).

    Marker:

    Article Title: Functional Dichotomy of Developmental Foxp3 + Treg Cell Subsets in the Visceral Adipose Tissue of Lean and Obese Mice
    Article Snippet: Viable cell numbers were determined using propidium iodide and a MACSQuant (Miltenyi Biotec). .. Prior to CD4 + T cell isolation from VAT via FACS, samples were depleted of lineage marker-positive cells (CD8a, CD11b, CD11c, CD14, and CD19) using biotin-conjugated mAbs, along with streptavidin-conjugated microbeads and the autoMACS Pro magnetic separation system (Miltenyi Biotec). .. Samples were stained with DRAQ7 (BioStatus) for dead cell exclusion, filtered through 40 μm cell strainers, and analyzed on an LSR Fortessa or sorted with a FACS Aria II or III (all BD).

    Adoptive Transfer Assay:

    Article Title: Aged regulatory T cells fail to control autoimmune lacrimal gland pathogenic CD4 + T cells.
    Article Snippet: CD25KO mice are a model of Sjögren disease.. CD25KO mice have severe inflammation and infiltrating lymphocytes to the lacrimal glands (LG).. Whether the pathogenicity of CD25KO CD4+ T cells can be controlled in vivo by Tregs is unknown.

    Single Cell:

    Article Title: Aged regulatory T cells fail to control autoimmune lacrimal gland pathogenic CD4 + T cells.
    Article Snippet: CD25KO mice are a model of Sjögren disease.. CD25KO mice have severe inflammation and infiltrating lymphocytes to the lacrimal glands (LG).. Whether the pathogenicity of CD25KO CD4+ T cells can be controlled in vivo by Tregs is unknown.



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    https://www.bioz.com/product/cd4+t+cell+isolation/CD4%2B+T+Cell+Isolation+Kit%2C+mouse/10__1158_slash_0008___5472__can___26___0274-132-10-18
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    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Control, Lysis, Flow Cytometry, Staining, In Vitro, Isolation, Incubation, Expressing, Comparison

    PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Analysis, Activation Assay, Immunohistochemistry, Expressing

    PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Tissue, Microinjection, Injection, Staining, Analysis, Expressing, Activity Assay